Title image: Cooper Thome, a doctoral candidate in the Department of Chemical and Biological Engineering, uses a new "acoustic pipette," which could someday be used to deliver swift听test results at the bedside with only a finger-prick of blood. (Credits: Patrick Campbell/CU 麻豆影院.)
Go to the doctor to provide a blood sample and you鈥檙e typically faced with a needle and syringe and hours or even days of waiting to get results back from a lab.
CU 麻豆影院 researchers hope to change that with a new handheld, sound-based diagnostic system able to deliver precise results in an hour with a mere finger prick of blood.
The team describes the system in a new paper published .
鈥淲e鈥檝e developed a technology that is very user friendly, can be deployed in various settings and provides valuable diagnostic information in a short time frame,鈥� said senior author Wyatt Shields, assistant professor in the Department of Chemical and Biological Engineering at CU 麻豆影院.
The findings come as scientists have been racing to democratize diagnostic testing, which can be hard for people in rural areas or developing countries to access and, in the case of blood tests, frightening for those averse to needles.
While existing rapid tests, known as lateral-flow assays,听like COVID tests or pregnancy tests, can provide a quick 鈥測es鈥� or 鈥渘o鈥� as to whether a specific biomarker or biomolecule in the blood or urine is present, they typically can鈥檛 say how much, and they aren鈥檛 sensitive enough to detect very small amounts.
Meanwhile, the gold standard for clinical blood tests, known as an enzyme-linked immunosorbent assay (ELISA), is highly sensitive and specialized enough to detect rare or scarce biomarkers but requires expensive equipment and complex techniques, and it can take hours or days for patients to receive results.
The authors acknowledge that听skepticism exists in the biosensing field听since the highly publicized downfall of Theranos Inc., which promised as far back as听2015 to detect hundreds of biomarkers with a drop of blood. Their invention works听differently, they said, and , it is based on systematic experiments and peer-reviewed research.听
The acoustic pipette uses sound waves to test for biomarkers in blood.听Photo by Patrick Campbell/CU 麻豆影院.
Cooper Thome, left, and Wyatt Shields in the lab. Photo by Patrick Campbell/CU 麻豆影院.
鈥淲hile what they claimed to do isn鈥檛 possible right now, a lot of researchers are hoping something similar will be possible one day,鈥� said first-author Cooper Thome, a doctoral candidate in Shields鈥� lab. 鈥淭his work could be a step toward that goal鈥攂ut one that is backed by science that anybody can access.鈥�
Using sound waves in a new way
Shields and听Thome set out to develop a tool that is simultaneously sensitive, highly portable and easy-to-use.
Their secret ingredients: tiny particles they call 鈥渇unctional negative acoustic contrast鈥� particles (fNACPs) and a custom-built, handheld instrument or 鈥渁coustic pipette鈥� that听delivers sound waves to the blood samples inside.
As part of his doctoral work, Thome designed the fNACPs (essentially cell-sized rubber balls)听to be customized with functional coatings so they recognize and capture a designated biomarker of interest, such as an infectious virus or a protein deemed a red flag for a brewing health problem. The particles also respond to the pressure from sound waves differently than blood cells. Thome designed the acoustic pipette to harness this unique response.
鈥淲e鈥檙e basically using sound waves to manipulate particles to rapidly isolate them from a really small volume of fluid,鈥� said Thome, who specializes in the study of 鈥渁coustofluidics.鈥� 鈥淚t鈥檚 a whole new way of measuring blood biomarkers.鈥�
When a small amount of blood is mixed with the custom particles and placed inside the acoustic pipette, sound waves force the particles to the side of a chamber where they are trapped inside while the rest of the blood is flushed out.
The remaining biomarkers, attached to the particles, are then labeled with fluorescent tags and hit with lasers to determine the amount present.
All this happens in under 70 minutes inside a device that can fit in the palm of a hand.
Matching the gold standard clinical test
鈥淚n our paper, we demonstrate that this pipette and particle system can offer the same sensitivity and specificity as a gold-standard clinical test can but within an instrument that radically simplifies workflows,鈥� said Shields, noting that this time could likely be reduced more with future refinements. 鈥淚t gives us the potential to perform blood diagnostics right at the patient鈥檚 bedside.鈥�
This could be particularly useful for assessing not only whether a patient has an infectious disease but also what their viral load is and how fast it is growing, he said. The device could also potentially play a role in measuring antibodies to determine whether someone needed a booster shot or not, testing for allergies or detecting proteins associated with certain cancers.
The听study is a proof of concept, and more research is necessary before the device could be commercialized. The authors听have worked with Venture Partners听to apply听for patents and are now exploring ways to make the technology work for multiple patients at once (which would be useful in mobile clinics in rural areas, for instance) or test for multiple biomarkers simultaneously.
鈥淲e think this has a lot of potential to address some of the longstanding challenges that have come from having to take a blood sample from a patient, haul it off to a lab and wait to get results back,鈥� said Shields.
