Publications

  • 131) Dohrmann, P.R., Yuan, Q, , Sutton, M.D., and McHenry, C.S. (2016) DNA Polymerase III, but not Polymerase IV, Must be Bound to tau-Containing DnaX Complex to Enable Exchange into Progressing Replication Forks. J. Biol. Chem. 291, 11727-11735.
  • 130) Dohrmann, P.R., Correa, R., Frisch R.L., Rosenberg, S.M., McHenry, C.S. (2016) 鈥淭he DNA polymerase III holoenzyme contains gamma and is not a trimeric polymerase.鈥 Nucl. Acids Res. 44, 1285-1297.
  • 129) Lindow, J.C., Dohrmann, P.R., McHenry, C.S. (2015) 鈥淒NA Polymerase alpha Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Screen鈥澨J. Biol. Chem. 290, 16851-16860J. Biol. Chem.听290, 16851-16860.听听
  • 128) Manhart, C.M., McHenry, C.S. (2015) 鈥淚dentification of Subunit Binding Positions on a Model Fork and Displacements that Occur During Sequential Assembly of the E. coli Primosome.鈥澨J. Biol. Chem.听290, 10828-10839.
  • 127) McHenry, C.S. (2014)听鈥淐hemical biology of DNA replication.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)听Molecular life sciences:听an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_133-1.
  • 126) McHenry, C.S. (2014) 鈥淢any Bacteria use a Special Mutagenic Pol III in Place of Pol V.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)Molecular life sciences: an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_124-1.
  • 125) McHenry, C.S. (2014) 鈥淒naX Complex Composition and Assembly within Cells.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)听Molecular life sciences: an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_123-1.
  • 124) McHenry, C.S. (2014)听鈥淐ycling of the Lagging Strand Replicase During Okazaki Fragment Synthesis.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)听Molecular life sciences: an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_132-1
  • 123) McHenry, C.S. (2014) 鈥淒NA Polymerase III Structure.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)听Molecular life sciences: an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_131-1.
  • 122) McHenry, C.S. (2014)鈥淢echanism of Initiation Complex Formation.鈥 In: Bell E, Bond J, Klinman J, Masters B, Wells R (eds.)听 Molecular life sciences: an encyclopedic reference.听Springer Science+Business Media New York. DOI: 10.1007/978-1-4614-6436-5_128-1.
  • 121) Yuan, Q. and McHenry, C. (2014) Cycling of the听E. coli听lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.听Nucleic Acids Res.听42, 1747-1756.
  • 120) Antony, E., Weiland, E., Yuan, Q., Manhart, C.M., Nguyen, B., Kozlov, A.G., McHenry, C. and Lohmman, T.M.听(2013)听Multiple C-Terminal Tails within a Single听E. coli听SSB Homotetramer Coordinate DNA听Replication and Repair.听J. Mol. Biol.听425, 4802-4819.
  • 119) Ungermannova D., Lee J., Zhang G., Dallmann H.G., McHenry C. and Liu X. (2013)听High-Throughput Screening Alpha Screen Assay for Identification of Small-Molecule Inhibitors of Ubiquitin E3 Ligase SCFSkp2-Cks1.听J. Biomol Screen.,听8, 910-20.
  • 118) Seco, E., Zinder, J., Manhart, C.M., Piano, A.L., McHenry, C. and听Ayora, S. (2013) Bacteriophage SPP1听in vitro听DNA Replication Strategies Promote Viral and Disable Host Replication.听听Nucleic Acid Res.,听41, 1711-1721.
  • 117) Manhart, C.M. and听McHenry, C. (2013) The PriA Replication Restart Protein Blocks Replicase Access Prior to Helicase Assembly and Directs Template Specificity through its ATPase Activity.听听J. Biol. Chem.,听288,听3989-3999.
  • 116) McHenry, C. Bacterial DNA Replicases.听 E. Bell, J. Bond, J. Klinman, B. Siler Masters, and R. Wells (Eds.),Molecular Life Sciences: An Encyclopedic Reference.听Springer听Science+Business Media, New York.听DOI:听10.1007/978-1-4614-6436-5_54-2.
  • 115) Dohrmann, P.R., Manhart, C.M., Downey, C.D. and听McHenry, C. (2011) The Rate of Polymerase Release upon Filling the Gap between Okazaki Fragments is Inadequate to Support Cycling During Lagging Strand Synthesis. 听J. Mol. Biol.,听414,15-27.
  • 114) McHenry, C. (2011) Bacterial Replicases and Related Polymerases. 听Curr.Opin.Chem. Biol.,听15, 587-594.
  • 113) Downey, C.D., Crooke, E. and听McHenry, C. (2011) Polymerase Chaperoning and Multiple ATPase Sites Enable the听E. coli听DNA Polymerase III Holoenzyme to Rapidly Form Initiation Complexes. 听J. Mol. Biol.,听412,听340-353.
  • 112) McHenry, C. (2011)听听Breaking the Rules: Bacteria That Use Several DNA Polymerase IIIs.听EMBORep.听12, 408-414.
  • 111) McHenry, C. (2011) DNA Replicases from a Bacterial Perspective.听听Annu. Rev. Biochem.,听80, 403-436.
  • 110) Wieczorek, .A,听Downey, C.D., Dallmann, H.G. and听McHenry, C. (2010) Only one ATP-binding DnaX Subunit is Required for Initiation Complex Formation by the听Escherichia coli听DNA Polymerase III Holoenzyme. 听J. Biol. Chem.,听285, 29049-29053.
  • 109) Dallmann, H.G.,Fackelmayer, O.J., Tomer, G., Chen, J.Y., Wiktor-Becker, A., Ferrara, T., Pope, C., Oliveira, M.T., Burgers, P.M.J., Kaguni, L.K. and McHenry, C. (2010) Parallel Multiplicative Target Screening Against Divergent Bacterial Replicases: Identification of Specific Inhibitors with Broad Spectrum Potential.听Biochemistry,听49, 2551-2562.
  • 108) Downey, C.D. and McHenry, C. (2010) Chaperoning of a Replicative Polymerase onto a Newly-Assembled DNA Bound Sliding Clamp by the Clamp Loader.听Mol. Cell,听37,481-491.
  • 107) Sanders, G.M., Dallmann, H.G. and McHenry, C. (2010) Reconstitution of the听B. subtilis听Replisome with 13 Proteins including Two Distinct Replicases. 听Mol. Cell,听37, 273-281.
  • 106) Yuan,Q. and McHenry, C. (2009) Strand Displacement by DNA Polymerase III Occurs through a听tau-psi-chi听Link to SSB Coating the Lagging Strand Template. 听J. Biol. Chem.,听284, 31672-31679.
  • 105) Guiles, J., Sun, X., Critchley, I.A., Ochsner, U., Tregay, M., Stone, K., Bertino, J., Green, L., Sabin, R., Dean, F., Dallmann, H.G., McHenry, C. and听Janic, N. (2009) Quinazolin-2-ylamino-quinazolin-4-ols as Novel Non-nucleoside Inhibitors of Bacterial DNA Polymerase III.听听Bioorg Med Chem Lett.,听19, 800-802.
  • 104) Stano, N., Chen, J. and McHenry, C. (2006) A Coproofreading Zn+2-dependent Exonuclease within a Bacterial Replicase.听听Nat. Struct. Mol. Biol.,13, 458-459.
  • 103) Wieczorek, A. and McHenry, C. (2006)听The N-terminal php Domain of the alpha Subunit of the听E. coli听Replicase Binds the epsilon Proofreading Subunit.听听J. Biol. Chem.,听281,听12561-12567.
  • 102) Jarvis, T.C., Beaudry, A.A., Bullard, J.M., Ochsner, U., Dallmann, H.G. and听McHenry, C., (2005)听Discovery and Characterization of the Cryptic gamma Subunit of the Pseudomonad DNA Replicase. 听J. Biol. Chem.,听280, 40465-40473.
  • 101) Dohrmann, P.R. and听McHenry, C., (2005) A Bipartite Polymerase鈥揚rocessivity Factor Interaction: Only the Internal beta Binding Site of the alpha Subunit is Required for Processive Replication by the DNA Polymerase III Holoenzyme.听听听J. Mol. Biol.,听350, 228-239.
  • 100) Jarvis, T.C., Beaudry, A.A., Bullard, J.M., Janjic, N. and McHenry, C. (2005) Reconstitution of a minimal DNA Replicase from听Pseudomonas aeruginosa听and Stimulation by Non-cognate Auxiliary Factors.听听J. Biol Chem.,听280, 7890-7900.
  • 99)听 McHenry, C. (2003) Chromosomal Replicases as Asymmetric Dimers: Studies of Subunit Arrangement and Functional Consequences.听听Mol. Microbiol.,听49, 1157-1165.
  • 98)听 Bullard J.M., Williams J.C., Acker W.K., Jacobi C,. Janjic N. and McHenry C. (2002) DNA Polymerase III Holoenzyme from听Thermus thermophilus: Identification, Expression, Purification of Components and Use to Reconstitute a Processive Replicase.听听J. Biol. Chem.,听277, 13401-13408.
  • 97)听 Bullard, J., Pritchard, A.,听Song, M.-S., Glover, B., Wieczorek, A., Chen, J., Janjic, N. and McHenry, C. (2002)听 A Three Domain Structure for the delta Subunit of the DNA Polymerase III Holoenzyme delta听Domain III Binds delta'听and Assembles into the DnaX Complex. 听听J. Biol. Chem.,听277, 13246-13256.
  • 96)听 Muthuswami, R., Chen, J., Burnett, B., Thimmig, R., Janjic, N. and McHenry, C. (2002) The HIV Plus-Strand Transfer Reaction: Determination of Replication-Competent Intermediates and Identification of a Novel Lentiviral Element, the Primer Over-Extension Sequence.听听J. Mol. Biol.,听315, 311-323.
  • 95)听 Song, M-S. and McHenry, C. (2001)听 Carboxyl-terminal Domain III of the delta'听Subunit of DNA Polymerase III Holoenzyme Binds DnaX and Supports Cooperative DnaX-Complex Assembly. 听听J. Biol. Chem.,276, 48709-48715.
  • 94)听 Duan, C., Poticha, D., Stoeckli, T.C., Petropoulos, C.J., Whitcomb, J.M., McHenry, C. and Kuritzkes, D.R. (2001) Inhibition of Purified Recombinant Reverse Transcriptase from Wild-Type and Zidovudine-Resistant Clinical Isolates of Human Immunodeficiency Virus Type 1 by Zidovudine, Stavudine, and Lamivudine Triphosphates.听听J. Infect. Dis.,听184, 1336-1340.
  • 93)听 Song, M-S, Dallmann, H.G. and McHenry, C. (2001) Carboxyl-terminal Domain III of the delta' Subunit of the DNA Polymerase III Holoenzyme Binds delta.听J. Biol. Chem.,276, 40668-40679.
  • 92)听 Glover, B., Pritchard, A. and McHenry, C. (2001) Tau听Binds and Organizes听Escherichia coli听Replication Proteins through Distinct Domains:听 Domain III, shared by gamma听and tau,听oligomerizes DnaX. 听听J. Biol. Chem.,听276, 35842-35846.
  • 91)听 Pritchard, A.E. and McHenry, C. (2001)听 Assembly of听 DNA Polymerase III Holoenzyme:听 Co-assembly听 of gamma听and tau听is Inhibited by DnaX Complex Accessory Proteins but Stimulated by DNA polymerase III Core. 听听J. Biol. Chem.,276, 35217-35222.
  • 90)听 Song, M-S, Pham, P., Olson, M., Carter, J., Franden, M., Schaaper, R. and McHenry, C. (2001)听 The delta听and delta'听Subunits of the DNA Polymerase III Holoenzyme Are Essential for Initiation Complex Formation and Processive Elongation. 听听J. Biol. Chem.,276, 35165-35175.
  • 89)听 Glover, B. and McHenry, C. (2001)听 The DNA Polymerase III Holoenzyme:听 An Asymmetric Dimeric Replicative Complex with Leading and Lagging Strand Polymerases. 听听Cell,105, 925鈥934.
  • 88)听 Gao, D. and McHenry, C. (2001) Tau听Binds and Organizes听Escherichia coli听Replication Proteins through Distinct Domains:听听Domain III, shared by gamma and tau,听Binds delta-delta'听and chi-psi. 听听J. Biol. Chem.,276, 4447-4453.
  • 87)听 Gao, D. and McHenry, C. (2001) Tau听Binds and Organizes听Escherichia coli听Replication Proteins through Distinct Domains: 听Domain听IV, Located within the Unique听Carboxyl-terminus of tau,听Binds the听Replication听Fork听Helicase, DnaB.听听听J. Biol. Chem.,276, 4441-4446.
  • 86)听 Gao, D. and McHenry, C. (2001) Tau听Binds and Organizes听E. coli听Replication Proteins through Distinct Domains: Partial Proteolysis of Terminally Tagged tau to Determine Candidate Domains and to Assign Domain V as the alpha听Binding Domain. 听听J. Biol. Chem.,276, 4433-4440.
  • 85)听 Pritchard, A., Dallmann, G., Glover, B. and McHenry, C. (2000)听 A Novel Assembly Mechanism for the DNA Polymerase III Holoenzyme DnaX Complex:听 Association of delta-delta'听with DnaX4听Forms DnaX3delta-delta'.听听听EMBO J.,听19,听 6536-6545.
  • 84)听 Dallmann, H.G., Kim, S., Pritchard, A.E., Marians, K.J. and McHenry, C. (2000) Characterization of the Unique C-terminus of the听E. coli听tau DnaX Protein:听 Monomeric C-tau听binds alpha听and DnaB and can Partially Replace tau听in Reconstituted Replication Forks.听听J. Biol. Chem.,275, 15512-15519.
  • 83)听 Glover, B.P. and McHenry, C. (2000) The DnaX-binding Subunits delta'听and psi听Are Bound to gamma听and听Not tau听in the DNA Polymerase III Holoenzyme.听J. Biol. Chem.,275, 3017-3020.
  • 82)听 Pham, P.T., Olson, M.W., McHenry, C. and Schaaper, R.M. (1999) Mismatch Extension by听Escherichia coli听DNA Polymerase III Holoenzyme.听听J. Biol. Chem.,274, 3705-3710.
  • 81)听 Pritchard, A.E. and McHenry, C. (1999)听 Identification of the Acidic Residues in the Active Site of DNA Polymerase III.听听J. Mol. Biol.,285, 1067-1080.
  • 80)听 Glover, B.P. and McHenry, C. (1998) The chi-psi听Subunits of DNA Polymerase III Holoenzyme Bind to Single-stranded DNA-binding Protein (SSB) and Facilitate Replication of an SSB-coated Template.听听J. Biol. Chem.,听273, 23476-23484.听听
  • 79)听 Pham, P.T., Olson, M.W., McHenry, C. and Schaaper, R.M. (1998)听 The Base Substitution and Frameshift Fidelity of听Escherichia coli听DNA Polymerase III Holoenzyme听in Vitro.听J. Biol. Chem.,273, 23575-23584.
  • 78)听 Marians, K.J., Hiasa, H., Kim, D.R. and McHenry, C. (1998) Role of the Core DNA Polymerase III Subunits at the Replication Fork: alpha听Is the only Subunit Required for Processive Replication.听听J. Biol. Chem.,听273, 2452-2457.
  • 77)听 Kim, D. R., Pritchard, A.E. and McHenry, C. (1997) Localization of the Active Site of the alpha听Subunit of the听Escherichia coli听DNA Polymerase III Holoenzyme.听听J. Bacteriol.,听179, 6721-6728.听
  • 76)听 McHenry, C., Seville, M. and Cull, M.G. (1997) A DNA Polymerase III Holoenzyme-like Subassembly from an Extreme Thermophilic Eubacterium.听听J. Mol. Biol.,听272, 178-189.
  • 75)听 Burnett, B. and McHenry, C.听 (1997) Posttranscriptional Modification of Retroviral Primers is Required for Late Stages of DNA Replication.听听Proc. Natl. Acad. Sci., USA94, 7210-7215.
  • 74)听 Seville, M., West, A., Cull, M. and McHenry, C. (1996) Fluorometric Assay for DNA Polymerases and Reverse Transcriptase.听听BioTechniques,听21, 664-672.听
  • 73)听 Kim, S., Dallmann, H.G., McHenry, C. and Marians, K.J. (1996) Tau听Couples the Leading- and Lagging-strand Polymerases at the听Escherichia coli听DNA Replication Fork.听听J. Biol. Chem.,271, 21406-21412.
  • 72)听 Kim, D.R. and McHenry, C. (1996) Identification of the beta-Binding Domain of the alpha听Subunit of听Escherichia coli听Polymerase III Holoenzyme.听听J. Biol. Chem.,听271,听20699-20704.
  • 71)听 Kim, D.R. and McHenry,C. (1996) Biotin Tagging Deletion Analysis of Domain Limits Involved in Protein-Macromolecular Interactions:听 Mapping the tau Binding Domain of the DNA Polymerase III alpha听Subunit.听听J. Biol. Chem.,听271,听20690-20698.
  • 70)听 Kim, D.R. and McHenry, C. (1996)听In Vivo听Assembly of Overproduced DNA Polymerase III:听 Overproduction, Purification and Characterization of the alpha, alpha-epsilon听and alpha-epsilon-theta听Subunits.听听J. Biol. Chem.,听271,听20681-20689.
  • 69)听 Kim, S., Dallmann, H.G., McHenry, C. and Marians, K. J. (1996) Tau听Protects beta听in the Leading-strand Polymerase Complex at the Replication Fork.听听J. Biol. Chem.,听271, 4315-4318.
  • 68)听 Pritchard, A.E., Dallmann, H.G. and McHenry, C. (1996)听In Vivo听Assembly of the tau-Complex of the DNA Polymerase III Holoenzyme Expressed from a Five-Gene Artificial Operon:听 Cleavage of the tau-complex to Form a Mixed tau-gamma听complex by the OmpT Protease.听听J. Biol. Chem.,听271, 10291-10298.听
  • 67)听 Kim, S., Dallmann, H.G., McHenry, C. and Marians, K.J. (1996) Coupling of a Replicative Polymerase and Helicase:听 A tau-DnaB Interaction Mediates Rapid Replication Fork Movement.听听Cell,84, 643-650.
  • 66)听 Olson, M., Dallmann, H.G. and McHenry, C. (1995) DnaX-Complex of听E. coli听DNA Polymerase III Holoenzyme:听 The chi-psi听Complex Functions by Increasing the Affinity of tau听and gamma听for delta-delta'听to a Physiologically Relevant Range.听听J. Biol. Chem.,听270, 29570-29577.
  • 65)听 Dallmann, H.G. and McHenry, C. (1995) DnaX-Complex of听Escherichia coli听DNA Polymerase III Holoenzyme:听 Physical Characterization of the DnaX Subunits and Complexes.听听J. Biol. Chem.,听270, 29563-29569.
  • 64)听 Dallmann, H.G., Thimmig, R. and McHenry, C. (1995) DnaX-Complex of听E. coli听DNA Polymerase III Holoenzyme:听 Central Role of tau听in Initiation Complex Assembly and in Determining the Functional Asymmetry of Holoenzyme.听听J. Biol. Chem.,听270, 29555-29562.
  • 63)听 Cull, M. and McHenry, C. (1995) Purification of听E. coli听DNA Polymerase III Holoenzyme, in听DNA ReplicationMethods in Enzymology, Vol. 262, Chapter 3 (Campbell, J., volume ed.), pp 22-35, Academic Press, New York.
  • 62)听 Reems, J., Wood, S. and McHenry, C. (1995)听E. coli听DNA Polymerase III Holoenzyme Subunits alpha, beta and gamma听Directly Contact the Primer Template.听听J. Biol Chem.,听270, 5606-5613.
  • 61)听 Reems, J. and McHenry, C. (1994)听E. coli听DNA Polymerase III Holoenzyme Footprints Three Helical Turns of Its Primer.听听J. Biol. Chem.,听269, 33091-33096.
  • 60)听 You, J-C. and McHenry, C. (1994) HIV Nucleocapsid Protein Accelerates Strand Transfer of the Terminally Redundant Sequences Involved in Reverse Transcription.听听J. Biol. Chem.,听269, 31491-31495.
  • 59)听 Hottiger, M., Podust, V., Thimmig, R., McHenry, C. and H眉bscher, U. (1994) Strand Displacement Activity of the Human Immunodeficiency Virus Type 1 Reverse Transcriptase Heterodimer and Its Individual Subunits.听听J. Biol. Chem.,听269, 986-991.
  • 58)听 Carter, J., Franden, M., Lippincott, J. and McHenry, C. (1993) Identification, Molecular Cloning and Characterization of the Gene Encoding the chi Subunit of DNA Polymerase III Holoenzyme of听Escherichia coli.听Mol. Gen. Genet.,241, 399-408.
  • 57)听 Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1993) Identification, Isolation, and Overexpression of the Gene Encoding the psi听Subunit of DNA Polymerase III Holoenzyme.听听J. Bacteriol.,175, 5604-5610.
  • 56)听 Thimmig, R. and McHenry, C. (1993) HIV Reverse Transcriptase:听 Expression in听E. coli, Purification and Characterization of a Functionally and Structurally Asymmetric Dimeric Polymerase.听听J. Biol. Chem.,268, 16528-16536.听听
  • 55)听 You, J-C. and McHenry, C. (1993) HIV Nucleocapsid Protein: Expression in听E. coli, Purification and Characterization.听听J. Biol. Chem.,268, 16519-16527.
  • 54)听 Carter, J., Franden, M., Aebersold, R., Kim, D-R. and McHenry, C. (1993) Isolation, Sequencing and Overexpression of the Gene Encoding the theta Subunit of DNA Polymerase III Holoenzyme.听听Nucleic Acids Res.,21, 3281-3286.
  • 53)听 Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1993) Identification, Isolation and Characterization of the Structural Gene Encoding the delta' Subunit of听E. coli听DNA Polymerase III Holoenzyme.听听J. Bacteriol.,175, 3812-3822.
  • 52)听 Carter, J., Franden, M., Aebersold, R. and McHenry, C. (1992) Molecular Cloning, Sequencing and Overexpression of the Structural Gene Encoding the delta听Subunit of听E. coli听DNA Polymerase III Holoenzyme.听听J. Bacteriol.,174, 7013-7025.
  • 51)听 Wu, C.A., Zechner, E.L., Reems, J., McHenry, C., and Marians, K.J. (1992) Coordinated Leading- and Lagging-strand Synthesis at the听Escherichia coli听DNA Replication Fork: V.听 Primase Action Regulates the Cycle of Okazaki Fragment Synthesis.听听J. Biol. Chem.,267, 4074-4083.
  • 50)听 Wu, C.A., Zechner, E.L., Hughes, A.J.,Jr., Franden, M., McHenry, C. and Marians, K.J.听 (1992) Coordinated Leading- and Lagging-strand Synthesis at the听Escherichia coli听DNA Replication Fork: IV.听 Reconstitution of an Asymmetric, Dimeric DNA Polymerase III Holoenzyme.听听J. Biol. Chem.,267, 4064-4073.
  • 49)听 Griep, M. A. and McHenry, C. (1992) Fluorescence Energy Transfer Between the Primer and the beta听Subunit of the DNA Polymerase III Holoenzyme .听J. Biol. Chem.,267, 3052-3059.
  • 48)听 McHenry, C. (1991) DNA Polymerase III Holoenzyme:听 Components, Structure and Mechanism of a True Replicative Complex.听听J. Biol. Chem.,266, 19127-19130.
  • 47)听 McHenry, C. (1992) DNA Replication (invited article) in听Emerging Targets for Antibacterial and Antifungal Chemotherapy,听(J. Sutcliffe and N. Georgopapadakou, eds.), pp. 37-67, Chapman & Hall, New York.
  • 46)听 Flower, A. and McHenry, C., (1991) Transcriptional Organization of the听Escherichia coli dnaX听Gene.听J. Mol. Biol.,听220, 649-658.
  • 45)听 Hughes, A., Bryan, S., Chen, H. Moses, R. and McHenry, C.听 (1991)听听Escherichia coli听DNA Polymerase II is Stimulated by DNA Polymerase III Holoenzyme Auxiliary Subunits.听听J. Biol. Chem.,听266, 4568-4573.
  • 44)听 Reems, J., Griep, M. and McHenry, C. (1991)听 The Proofreading Activity of DNA Polymerase III Responds Like Elongation Activity to Auxiliary Subunits.听听J. Biol. Chem.,听266, 4878-4882.
  • 43)听 Griep, M. and McHenry, C. (1990)听 Dissociation of the DNA Polymerase III Holoenzyme beta2听Subunits is Accompanied by a Conformational Change at Distal Cysteines 333.听听J. Biol. Chem.,听265, 20356-20363.
  • 42)听 Griep, M. Reems, J., Franden, M. and McHenry, C. (1990)听 Reduction of the Potent DNA Polymerase III Holoenzyme 3'-->听5' Exonuclease Activity by Template-Primer Analogs.听听Biochemistry,听29, 9006-9014.
  • 41)听 Flower, A. and McHenry, C. (1990)听 The gamma听Subunit of DNA Polymerase III Holoenzyme of听Escherichia coli听is Produced by Ribosomal Frameshifting.听Proc. Natl. Acad. Sci. U.S.A.,87, 3713-3717.
  • 40)听 McHenry, C., Griep, M. Tomasiewicz, H. and Bradley, M. (1989) Functions and Regulation of DNA Polymerase III Holoenzyme, An Asymmetric Dimeric DNA Polymerase, in听Molecular Mechanisms in DNA Replication and Recombination听(C. Richardson and I.R. Lehman, eds.), pp. 115-126, Alan R. Liss, Inc., New York.
  • 39)听 Griep, M. and McHenry, C. (1989) Glutamate Overcomes the Salt Inhibition of DNA Polymerase III Holoenzyme.听听J. Biol. Chem.,听264, 11294-11301.
  • 38)听 Cull, M. and McHenry, C. (1990) Preparation of Extracts from Prokaryotes in Guide to Protein Purification, in听Methods in Enzymology,听182, Chapter 12 (volume Deutscher, M., ed.), pp. 147-154, Academic Press, New York.听听
  • 37)听 McHenry, C. (1988)听 The Asymmetric Dimeric Polymerase Hypothesis:听 A Progress Report. 听听Biochem. Biophys. Acta听(invited review),听951, 240-248.
  • 36)听 McHenry, C. (1989)听 Replication of the Human Immunodeficiency Virus in听MolecularBiology of Chromosome Function听(K. Adolph, ed.), Ch. 5, pp. 97-129, Springer-Verlag, New York.
  • 35)听 Franden, M. and McHenry, C. (1988)听 Detection of an Epitope, Not Required for Polymerization, That is Conserved between听E.听coli DNA Polymerases I and III and Bacteriophage T4 DNA Polymerase.听Nucleic Acids Res.,16, 6353-6360.听听
  • 34)听 Griep M. and McHenry, C. (1988) The Dimer of the beta听Subunit of听Escherichia coli听DNA polymerase III Holoenzyme is Dissociated into Monomers upon Binding Magnesium(II).听听Biochemistry,听27, 5210-5215.
  • 33)听 McHenry, C.,听Tomasiewicz, H., Griep, M., F眉rste, P. and Flower, A. (1988)听 DNA Polymerase III Holoenzyme:听 Mechanism and Regulation of a True Replicative Complex in听DNA Replication and Mutagenesis听(Moses, R and Summers, W., eds.), pp. 14-26, American Society for Microbiology, Washington, D.C.
  • 32)听 McHenry, C. (1988) DNA Polymerase III Holoenzyme of听Escherichia coli.听Ann. Rev. Biochem.,57, 519- 550.
  • 31)听 Santi, D., McHenry, C., Raines, R. and Ivanetich, K. (1987) Kinetics and Thermodynamics of the Interaction of 5-Fluoro-2'-Deoxyuridylate with Thymidylate Synthase.听听Biochemistry,听26, 8606-8613.
  • 30)听 McHenry, C., Flower, A.M. and Hawker, J.R. (1988) Coordination of Leading with Lagging Strand Synthesis by the Asymmetric Dimeric DNA Polymerase III Holoenzyme of听E. coli,听in听Cancer Cells 6: Eukaryotic DNA Replication听(Kelly, T. and Stillman, B., eds.), pp. 35-41, Cold Spring Harbor, New York.
  • 29)听 Hawker, J.R. and McHenry, C. (1987)听 Monoclonal Antibodies Specific for the tau听Subunit of the DNA Polymerase III Holoenzyme of听Escherichia coli:听 Use to Demonstrate that tau听is the Product of the听dnaZX Gene and that both it and gamma,听the听dnaZ Gene Product, are Integral Components of the Same Enzyme Assembly.听听J. Biol. Chem.,听262, 12722-12727.
  • 28)听 Tomasiewicz, H. and McHenry, C. (1987)听 Sequence Analysis of the听Escherichia colidnaE Gene.听J. Bacteriol.,听169, 5735-5744.
  • 27)听 Oberfelder, R. and McHenry, C. (1987) Characterization of 2鈥 (3鈥)-Trinitrophenyl-ATP as an Inhibitor of ATP-dependent Initiation Complex Formation between the DNA Polymerase III Holoenzyme and Primed DNA.听听J. Biol. Chem.,听262, 4190-4194.
  • 26)听 Flower, A. and McHenry, C. (1986)听 The Adjacent听dnaZ and听dnaX Genes of听Escherichia coli听are Contained within One Continuous Open Reading Frame.听Nucleic Acids Res.,听14,听8091-8101.
  • 25)听 Kwon-Shin, O., Bodner, J., McHenry, C. and Bambara, R. (1987)听 Properties of Initiation Complexes Formed between听Escherichia coli听DNA Polymerase III Holoenzyme and Primed DNA in the Absence of ATP.听听J. Biol. Chem.,听262,听2121-2130.
  • 24)听 McHenry, C., Oberfelder, R., Johanson, K., Tomasiewicz, H. and Franden, M. (1987)听 Structure and Mechanism of the DNA Polymerase III Holoenzyme, in听DNA Replication and Recombination听(Kelly, T. and McMacken, R., eds.),听 pp 47-61, Alan R. Liss, Inc., New York.
  • 23)听 Johanson, K., Haynes, T. and McHenry, C. (1986)听 Chemical Characterization and Purification of the beta听Subunit of the DNA Polymerase III Holoenzyme from an Overproducing Strain. 听听J. Biol. Chem.,261, 11460-11465.
  • 22)听 LaDuca, R., Crute, J., McHenry, C. and Bambara, R. (1986)听 The beta听Subunit of the听Escherichia coli听DNA Polymerase III Holoenzyme Interacts Functionally with the Catalytic Core in the Absence of other Subunits. 听听J. Biol. Chem.,听261, 7550-7557.
  • 21)听 McHenry, C. (1985) DNA Polymerase III Holoenzyme of听Escherichia coli: Components and Function of a True Replicative Complex. (invited review).听听Mol. Cell. Biochem.,66, 71-85.
  • 20)听 Wu, Y.H., Franden, M., Hawker, J.R. and McHenry, C. (1984) Monoclonal Antibodies Specific for the alpha听Subunit of the听Escherichia coli听DNA Polymerase III Holoenzyme. 听听J. Biol. Chem.,听259, 12117-12122.
  • 19)听 Shepard, D., Oberfelder, R. Welch, M. and McHenry, C. (1984) Determination of the Precise Location and Orientation of the听Escherichia coli dnaE Gene. 听听J. Bacteriol.,听158, 455-459.
  • 18)听 McHenry, C. and Johanson, K. (1984) DNA Polymerase III Holoenzyme of听Escherichia coli:听 An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases in听Proteins Involved in DNA Replication, (H眉bscher, U., and Spadari, S., eds.), pp 315-319,听Plenum Press.
  • 17)听 Johanson, K. and McHenry, C. (1984) Adenosine 5'-0-(3-thiotriphosphate) Can Support the Formation of an Initiation Complex between the DNA Polymerase III Holoenzyme and Primed DNA.听听J. Biol. Chem.,听259, 4589-4595.
  • 16)听 La Duca, R., Fay, P., Chuang, C., McHenry, C. and Bambara, R. (1983) Site-Specific Pausing of DNA Synthesis Catalyzed by Four Forms of听Escherichia coli听DNA Polymerase III.听听Biochemistry,听22, 5177-5188.
  • 15)听 Crute, J., La Duca, R., Johanson, K., McHenry, C. and Bambara, R. (1983) Excess beta听Subunit Can Bypass the ATP Requirement for Highly Processive Synthesis by the听Escherichia coli听DNA Polymerase III Holoenzyme.听听J. Biol. Chem.,听258, 11344-11349.
  • 14)听 Welch, M. and McHenry, C. (1982) Cloning and Identification of the Product of the听dnaE Gene of听Escherichia coli.听J. Bacteriol.,听152, 351-356.听听
  • 13)听 Johanson, K. and McHenry, C. (1982) The beta听Subunit of the DNA Polymerase III Holoenzyme Becomes Inaccessible to Antibody after Formation of an Initiation Complex with Primed DNA. 听听J. Biol. Chem.,听257, 12310-12315.
  • 12)听 Fay, P., Johanson, K., McHenry, C. and Bambara, R. (1982) Size Classes of Products Synthesized Processively by Two Subassemblies of听Escherichia coliDNA Polymerase III Holoenzyme. 听听J. Biol. Chem.,听257, 5692-5699.
  • 11)听 McHenry, C. (1982) Purification and Characterization of听Escherichia coli听DNA Polymerase III': Identification of tau听as a Subunit of the DNA Polymerase III Holoenzyme. 听听J. Biol. Chem.,听257, 2657-2663.听
  • 10)听 Johanson, K. and McHenry, C (1981) Role of the beta听Subunit of the听Escherichia coli听DNA Polymerase III Holoenzyme in the Initiation of DNA Elongation in听Structure and DNA - Protein Interactions of Replication Origins听(D. Ray, ed.), pp. 425-436, Academic Press, New York.
  • 9)听听 McHenry, C. and Kornberg, A. (1981) DNA Polymerase III Holoenzyme, in听The Enzymes,听Nucleic Acids, Vol. 14, Pt. A, Ch. 3 (P. Boyer, ed.), pp. 39-50, Academic Press, New York.
  • 8)听听 McHenry, C. (1980) Multiple Forms of DNA Polymerase III of听Escherichia coli听in:听听Mechanistic Studies of DNAReplication and Genetic Recombination听(B. Alberts, ed.), pp. 569-577, Academic Press, New York.
  • 7)听听 Fay, P. Johanson, K., McHenry, C. and Bambara, R. (1981) Size Classes of Products Synthesized Processively by DNA Polymerase III and DNA Polymerase III Holoenzyme of听Escherichia coli. 听听J. Biol. Chem.,听256, 976-983.
  • 6)听听 Johanson, K. and McHenry, C. (1980) Purification and Characterization of the beta听subunit of the DNA Polymerase III Holoenzyme of听Escherichia coli.听J. Biol.Chem.,听255, 10984-10990.
  • 5)听听 McHenry, C. and Crow, W. (1979) DNA Polymerase III听Escherichia coli: Purification and Identification of Subunits. 听听J. Biol. Chem.,254, 1748-1753.
  • 4)听听 McHenry, C. and Kornberg, A. (1977) DNA Polymerase III Holoenzyme of听Escherichia coli:听 Purification and Resolution into Subunits. 听听J. Biol.听Chem.,252, 6478-6484听and correction of publishers inversion, left to right, of figure 3, vol 253, p 645.
  • 3)听听 Santi, D., McHenry, C. and Sommer, H. (1974) Mechanism of Interaction of Thymidylate Synthetase with 5-Fluorodeoxyuridylate. 听听Biochemistry,13, 471-481.
  • 2)听听 Santi, D., McHenry, C. and Perriard, E. (1974) A Filter Assay for Thymidylate Synthetase Using 5-Fluoro-2'-deoxyuridylate as an Active Site Titrant.听听Biochemistry,13, 467-470.听听
  • 1)听听 Santi, D. and听McHenry, C. (1972) 5-Fluorodeoxyuridylate:听 Covalent Complex with Thymidylate Synthetase. 听Proc. Nat. Acad. Sci.,听69, 1855-1857.