Otto Kletzien likes to stay busy. He鈥檚 a Signaling and Cellular Regulation (SCR) fellow, one of approximately 15 in the department, an active participant and former leader of CU 麻豆影院鈥檚 RNA Club chapter, for which he ran operations and finance, a contributor to the Synthetic Biology Journal Club, and he鈥檚 a proud dog dad. Join us to learn more about Otto鈥檚 life as a research-focused CU Biochem graduate student.
Discovery and Innovation in RNA Research
聽聽聽聽聽聽聽聽聽聽聽聽聽聽聽 Otto鈥檚 longstanding interest in RNA research landed him in the right place for pursuing graduate school. Colorado鈥檚 celebrated history of RNA research has roots right here at CU 麻豆影院. Seminal discoveries include shedding light on the biocatalytic functions of RNA, characterizing enzymes for synthesizing and modifying RNA, and developing novel techniques for the identification of RNA ligands and the chemical synthesis of RNA. Otto hopes to continue this legacy of discovery and innovation through his work on RNA-protein complexes and riboswitches in the Batey lab. 鈥淩ob鈥檚 [Batey] lab was my first choice. He liked riboswitch work, studying the structure and designing and engineering RNA for specific applications.鈥� It wasn鈥檛 just Batey鈥檚 research that caught his eye, 鈥淚 also felt like I fit into the lab environment. We have a similar sense of humor and we have fun.鈥� Batey鈥檚 lab also allows for flexibility and autonomy in a largely graduate student-driven environment, and Otto has enjoyed taking project ideas in new directions in collaboration with peers and other faculty both intra- and inter-departmentally.聽
Day to day, you鈥檒l likely find Otto working in vitro, running electrophoresis gels, or conducting an RNA assay. This means quite of bit of calculation, perfecting bioinformatic pipelines, and optimizing assay panels. His thesis focuses on identifying RNA features that drive affinity for RNA-binding proteins, in order to better understand why they are biologically relevant. 鈥淭his protein domain that recognizes RNA is very abundant, but poorly understood and I want to figure out more about what sort of RNA features they recognize.鈥� In addition to his graduate thesis, Otto enjoys having the flexibility to explore other areas by contributing to different projects in the lab. As a side project, he鈥檚 adding to work that piques his early interests in riboswitches.
Otto鈥檚 interest in RNA research has also led him to join and take on a leadership role in the CU 麻豆影院 RNA Club. The club was founded in the 1980鈥檚 and continues to bring together researchers from CU 麻豆影院, CU Denver Anschutz, and Colorado State to discuss current RNA research by grad students, post-docs, and faculty. 鈥淚t was an opportunity for me to stay on top of RNA research, meet others, and make some good friends.鈥� Although Otto has moved on, RNA club still meets bi-monthly and is always looking for new members!
Turning the Research Corner
Otto has been working on expanding his research repertoire by also learning to code. 鈥淲e [Rob and I] always planned for me to do some bioinformatics training.鈥� Taking advantage of training opportunities to build this skillset proved valuable to Otto鈥檚 professional development and opened up new avenues for his lab work. Like many biochemists, Otto didn鈥檛 take computer science courses as an undergraduate, so he took advantage of continuing education resources offered by the department. There鈥檚 a workshop every summer on next-generation sequencing run by Robin Dowell and Mary Ellen through BioFrontiers for grad students and postdocs: 鈥淭he ENCODE consortium generates lots of bioinformatics data, so just learning how to use聽command line and figuring out the basic programs for quality control checks, gathering raw data, finding things that are conserved and filter out background.鈥� Otto鈥檚 hands-on experience working with these systems has not only helped him take his research in new directions, but also means a stronger resume, and tools that he will undoubtedly continue to use in the future. 鈥淎 lot of the labs that make this software don鈥檛 make money. They don鈥檛 get paid for the software so it鈥檚 less likely to be user friendly,鈥� which is why opportunities to learn the fundamentals from experts with institutional knowledge within the department are聽so critical.
In addition to the summer training program run by Biofrontiers, CU offers myriad resources for graduate students to learn programming and data science skills including a new Compuational Biology Minor that聽teaches undergraduates to combine computational thinking and algorithms to study biological problems and systems.
Getting into Research
Spending time purifying proteins in a lab as an undergrad was an important skill-building period. Otto also worked in the pharmaceutical industry for a year-long enough to learn how to organize and manage data聽and to firm up聽his research interests. This period fomented an interest in higher-level research, initially in bioengineering, which has since led Otto to the groundbreaking research he鈥檚 now conducting. Otto recommends getting into a lab, conducting research, and learning how to follow through on a project. Joining a lab as an undergrad set up the skills he uses today. In the Batey lab, Otto says the undergrads he works alongside are involved in every part of the protocol including creating libraries, running activity assays, and contributing to the data pipeline. If you鈥檙e an undergraduate interested in getting into a lab of your own, reach out to CU Biochem鈥檚 undergraduate program manager.聽
Batey Lab - BCHM聽 聽 聽 聽SCR - BCHM聽 聽 聽 聽Undergrad Research - BCHM聽 聽 聽聽聽IQ Biology聽-聽Biofrontiers聽 聽 聽 聽聽 聽 聽聽聽Comp Bio Minor
