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Laser Studies of Spectroscopy, Reaction Dynamics and Single Molecule Microscopy: Chemical Physics, Nanomaterials and Biophysics

The Nesbitt Laboratory pursues research in four main areas:

The group's work involves extensive use of state-of-the art cw and ultrafast pulsed laser technology, nonlinear generation of tunable mid and near-infrared laser light, fast analog electronics, scan probe methods, confocal microscopy, servo-loop control, shaped supersonic expansions, plasma discharges, kinetic analysis and ab initio quantum theoretical calculations. A central unifying goal of the research program is the elucidation of fundamental kinetics and dynamics of elementary chemical/biophysical processes from both experimental and theoretical perspectives.

Professor Nesbitt is a , a Physicist at the National Institute of Standards and Technology (), a Full Professor in the  and a Lecturer in the .

N. F. Dupuis, E. D. Holmstrom and D. J. Nesbitt, “Molecular crowding effects on single molecule RNA folding/unfolding thermodynamics and kinetics,” Proc. Nat. Acad. Sci, 111, 8464-8469,  (2014).

E. D. Holmstrom and D. J. Nesbitt, “Single-molecule fluorescence resonance energy transfer studies of the human telomerase RNA psuedoknot: Temperature/urea dependent folding kinetics and thermodynamics,” J. Phys. Chem. B 118, 3853-3863, (2014).

E. D. Holmstrom, J. T. Polaski, R. T. Batey, and D. J. Nesbitt, “Single-molecule conformational dynamics of a biologically functional hydroxocobalamin riboswitch,” J. Am. Chem. Soc. 136, 16832-16843 (2014).

Y. Liu, E. Holmstrom, J. Zhang, P. Yu, J. Wang, M. A. Dyba, D. Chen, J. Ying, S. Lockett, D. J. Nesbitt, A. Ferre-D’Amare, R. Sousa, J. R. Stagno, and Y.-X. Wang, “Synthesis and applications of RNAs with position-selective labelling and mosaic composition,” Nature 522, 368-372, (2015)

E. D. Holmstrom, N. F. Dupuis, and D. J. Nesbitt, “Kinetic and thermodynamic origins of osmolyte-influenced nucleic acid folding,” J. Phys. Chem. B. 119, 3687–3696, (2015)

C.-H. Chang and D. J. Nesbitt, “Spectroscopy and dynamics of jet cooled polyynes in a slit supersonic discharge: Sub-Doppler infrared studies of diacetylene HCCCCH,” J. Phys. Chem. A. 119, 7040 -7950, DOI:10.1021/acs.jpca.5b02310 (2015).

C.-H. Chang and D. J. Nesbitt, “Sub-Doppler Infrared Spectroscopy of Propargyl

Radical (H₂CCCH) in a Slit Supersonic Expansion,” 142, 244313, (2015).

E. D. Holmstrom and D. J. Nesbitt, “Biophysical Insights from Temperature-Dependent Single-Molecule Förster Resonance Energy Transfer,” Ann. Rev. Phys.Chem.67, 1-747, (2016).

C.-H. Chang, J. Agarwal, W. D. Allen, and D. J. Nesbitt, “Sub-Doppler infrared spectroscopy and reaction dynamics of triacetylene in a slit supersonic expansion,” J. Chem. Phys. 144, 074301, (2016)

J. T. Polaski, E. D. Holmstrom, D. J. Nesbitt, and R. T. Batey, “Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch,” Cell Reports 15, 1100-1110, (2016).

A.  L. Efros and D. J. Nesbitt, Nature Nanotech. 11, 661-671, (2016).

C. H. Hoffman and D. J. Nesbitt, “Quantum State Resolved 3D Velocity Map Imaging of Surface-Scattered Molecules: Incident Energy Effects in HCl + Self-Assembled Monolayer Collisions,” J. Phys. Chem. C. 120, 16687–16698, DOI: 10.1021/acs.jpcc.6b03973 (2016).

A. Sengupta, H.-L. Sung and D. J. Nesbitt, “Amino Acid Specific Effects on RNA Tertiary Interactions: Single-Molecule Kinetic and Thermodynamic Studies,” J. Phys. Chem. B 120, 10615-10627, (2016).

A. Zutz and D. J. Nesbitt, “Angle-Resolved Molecular Beam Scattering of NO at the Gas- Liquid Interface,” J. Chem. Phys. 147, 054704 (2017).

M. P. Ziemkiewicz, C. Pluetzer, J. Loreau, A. van  der Avoird, and D. J. Nesbitt, “Nuclear Spin/Parity Dependent Spectroscopy and Predissociation Dynamics in v_OH = 2 <— 0 Overtone Excited Ne-H₂O clusters: Theory and Experiment,” J. Chem. Phys. (2017).

A. Kortyna, A. J. Samin, T. A. Miller and D. J. Nesbitt “Sub-Doppler infrared spectroscopy of resonance-stabilized hydrocarbon intermediates: ν₃ (b₂) and ν₄ (a₁) CH stretch modes and CH₂ rotor tunneling dynamics of benzyl radical,” Phys. Chem. Chem. Phys. 19, 29812-298821 (2017).